Composite
Part:BBa_K3285669
Designed by: PRANAV S R Group: iGEM19_IISER-Pune-India (2019-10-16)
Lead biosensor with improved transcription efficiency for better GFP readout.
The biobrick BBa_K1758333 is a lead biosensor that detects lead in a sample making use of the pbrR-pbrAP repressible system. However with characterization of this part, we realized the sfGFP output is not very strong and consistent. Through modelling, we found out that using an RBS in between pbrAP and sfGFP of lower efficiency will improve those factors.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 465
Illegal NheI site found at 488 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 655
[edit]
Categories
Parameters
None |