Composite

Part:BBa_K3285669

Designed by: PRANAV S R   Group: iGEM19_IISER-Pune-India   (2019-10-16)


Lead biosensor with improved transcription efficiency for better GFP readout.

The biobrick BBa_K1758333 is a lead biosensor that detects lead in a sample making use of the pbrR-pbrAP repressible system. However with characterization of this part, we realized the sfGFP output is not very strong and consistent. Through modelling, we found out that using an RBS in between pbrAP and sfGFP of lower efficiency will improve those factors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 465
    Illegal NheI site found at 488
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 655


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Parameters
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